個(gè)人簡(jiǎn)介

北京大學(xué)生命科學(xué)學(xué)院教授

教育經(jīng)歷

1984年獲得中國(guó)科技大學(xué)生物系分子生物學(xué)專業(yè)學(xué)士學(xué)位；1992年獲得愛爾蘭國(guó)立大學(xué)科克學(xué)院微生物學(xué)系博士學(xué)位；

研究方向

多年來(lái)，本實(shí)驗(yàn)室的工作得到國(guó)際同行的認(rèn)可，與歐洲的幾個(gè)實(shí)驗(yàn)室建立并維持著卓有成效的合作關(guān)系，如法國(guó)巴斯德研究所、英國(guó)帝國(guó)理工學(xué)院、英國(guó)John Innes研究所、愛爾蘭Carlow理工學(xué)院、愛爾蘭國(guó)立大學(xué)Cork學(xué)院、西班牙國(guó)家生物技術(shù)中心、德國(guó)比勒菲爾德大學(xué)等。主要得到國(guó)家自然科學(xué)基金、國(guó)家科技部“863”、“973”項(xiàng)目基金、中法先進(jìn)合作項(xiàng)目、國(guó)家教育部基金資助。主要工作包括：大腸桿菌及相關(guān)細(xì)菌中的基因調(diào)控網(wǎng)絡(luò)，尤其是碳代謝和氮代謝的調(diào)控偶聯(lián)；大腸桿菌及相關(guān)細(xì)菌中的基因調(diào)控機(jī)理；植物與微生物相互作用的分子生物學(xué)及功能基因組學(xué)研究；生物修復(fù)領(lǐng)域的研究（功能基因的分離）；在大腸桿菌及相關(guān)細(xì)菌中大量表達(dá)外源基因技術(shù)等。取得的主要成就有，發(fā)現(xiàn)原核基因表達(dá)調(diào)控中碳代謝及氮代謝之間的新的偶聯(lián)作用及其分子機(jī)理；首次發(fā)現(xiàn)“下游激活序列”對(duì)固氮基因的調(diào)控作用；獲得了新型高效抗草苷膦基因，在核酸水平上與已知基因沒有同源性，在氨基酸水平上也只有37％左右的同源性，具有與受專利保護(hù)的EPSPS基因保守區(qū)不同的序列特征。目前世界上70％的轉(zhuǎn)基因植物含有抗除草劑基因，其中60％是草甘膦耐受型EPSPS基因,具有很大的應(yīng)用前景。

代表性成果

1. Xiang, N., Guo, C., Liu, J., Xu, H., Dixon, R.,* Yang, J.,* and Wang, Y.P.* (2020) Using synthetic biology to overcome barriers to stable expression of nitrogenase in eukaryotic organelles. Proc Natl Acad Sci USA. 117 (28): 16537-16545. doi:10.1073/pnas.2002307117
2.Jiang, F., Li, N., Wang, X., Cheng, J., Huang, Y., Yang, Y., Yang, J., Cai, B., Wang, Y.P., Jin, Q., and Gao, N. (2019) Cryo-EM structure and assembly of an extracellular contractile injection system. Cell 177, 1–14. doi: 10.1016/j.cell.2019.02.020.
3. Yang, J., Xie, X., Xiang, N., Dixon, R.,* and Wang, Y.P.* (2018) A polyprotein strategy for stoichiometric assembly of nitrogen fixation components for synthetic biology. Proc Natl Acad Sci U S A. 201804992; DOI: 10.1073/pnas.1804992115 [Highlighted by Stefan Burén, Gema Ló pez-Torrejón, and Luis M. Rubio (2018) Extreme bioengineering tomeet the nitrogen challenge Proc Natl Acad Sci U S A. doi/10.1073/pnas.1812247115]
4. Zhu, M., Dai, X., Guo, W., Ge, Z., Yang, M., Wang, H., and Wang, Y.P.* (2017) Manipulating bacterial cell cycle and cell size by titrating the expression of ribonucleotide reductase. mBio 8: e01741-17..
5. Yang, J., Xie, X. Yang, M. Dixon, R.* and Wang, Y.P.* (2017) Modular electron transport chains from eukaryotic organelles function to support nitrogenase activity. Proc Natl Acad Sci U S A. 114: 3009-3011. doi:10.1073/pnas.1620058114 [Highlighted by Vicente, E.J. and Dean, D.R. Keeping the nitrogen-fixation dream alive. Proc Natl Acad Sci U S A. 2017, 114 (12): 3009-3011, doi:10.1073/pnas.1701560114. And by Good A. (2018) Toward nitrogen-fixing plants. Science 359 (6378): 869-870, DOI: 10.1126/science.aas8737]. It has been compiled by the Royal Society of Biology (UK) as one of The Big Biology Breakthroughs of 2017. https://www.rsb.org.uk/about-us/mysociety/158-biologist/features/1881-big-biology-breakthroughs-2017
6. Dai, X., Zhu, M., Warren, M., Balakrishnan, R., Patsalo, V., Okano, H., Williamson, J.R., Fredrick, K., Wang, Y.P.* Hwa, T.* Reduction of translating ribosomes enables Escherichia coli to maintain elongation rates during slow growth. Nature Microbiology 2016, 2:16231. doi: 10.1038/nmicrobiol.2016.231
7. Zhu, M., Dai, X.,* and Wang, Y.P.* Real time determination of bacterial in vivo ribosome translation elongation speed based on LacZ? complementation system. Nucleic Acids Res. 2016, 44 (20): e155. doi: 10.1093/nar/gkw698.
8. Tian, Z.X*., Yi, X.X., Cho, A., O`Gara, F., and Wang, Y.P.* CpxR activates MexAB-OprM efflux pump expression and enhances antibiotic resistance in both laboratory and clinical nalB-type isolates of Pseudomonas aeruginosa. PLOS Pathogens 2016, 12(10):e1005932. doi: 10.1371/journal.ppat.1005932.
9. Wang, J., Yan, D., Dixon, R.*, and Wang, Y.P.* Deciphering the principles of bacterial nitrogen dietary preferences: a strategy for nutrient containment. mBio 2016, 7(4):e00792-16. doi:10.1128/mBio.00792-16.
10. Yang, Y., Darbari, V.C, Zhang, N., Lu, D., Glyde, R., Wang, Y.P., Winkelman, J., Gourse, R.L., Murakami, K.S., Buck, M., Zhang, X.*, Structures of the RNA polymerase-σ54 reveal new and conserved regulatory strategies. Science, 2015, 349: 882-885 doi: 10.1126/science.aab1478