個人簡介

北京大學(xué)現(xiàn)代農(nóng)學(xué)院研究員。

教育經(jīng)歷

2011 - 2011 , 博士后 , 遺傳學(xué) , 美國普渡大學(xué)
2009 - 2010 , 博士后 , 遺傳學(xué) , 美國加州大學(xué)河濱分校
2007 - 2009 , 博士后 , 遺傳學(xué) , 美國康奈爾大學(xué)
2002 - 2007 , 理學(xué)博士 , 遺傳學(xué) , 中國科學(xué)院遺傳與發(fā)育生物學(xué)研究所
1998 - 2002 , 理學(xué)學(xué)士 , 生物學(xué) , 北京師范大學(xué)

研究方向

表觀遺傳學(xué)主要包括DNA甲基化、組蛋白修飾、非編碼RNA和染色質(zhì)的空間結(jié)構(gòu)等方面，在基因的表達調(diào)控、轉(zhuǎn)座子的沉默、和調(diào)控植物發(fā)育等方面起著重要作用。其中DNA甲基化的水平和動態(tài)調(diào)控主要由甲基化和去甲基化這兩個途經(jīng)來協(xié)同調(diào)控。在植物中對DNA甲基化途經(jīng)的研究已經(jīng)比較深入，但對于DNA甲基化下游參與基因沉默的表觀遺傳機制還不清楚。相對于DNA甲基化的研究，目前對DNA主動去甲基化的分子機制和生物學(xué)功能的認識還十分有限。此外，非編碼RNA尤其是長非編碼RNA的生物學(xué)功能還不清楚。我們將運用遺傳學(xué)，生物化學(xué)和細胞生物學(xué)的方法，對植物體內(nèi)DNA去甲基化的機制以及非編碼RNA的生物學(xué)功能進行深入的研究。主要研究方向包括：1、植物基因組DNA主動去甲基化的分子機制和生物學(xué)功能；2、植物基因沉默的表觀遺傳學(xué)機制；3、植物非編碼RNA-蛋白復(fù)合體的鑒定和功能分析。

代表性成果

1. Ren Z.*, Gou R.*, Zhuo W.*, Chen Z.*, Yin X., Cao Y., Wang Y., Mi Y., Liu Y., Wang Y., Fan L-M., Deng X.W., and Qian W. # (2023) The MBD-ACD DNA methylation reader complex recruits MICRORCHIDIA6 to regulate rRNA gene expression in Arabidopsis. Plant Cell. Koad313, https://doi.org/10.1093/plcell/koad313.

2. Li J. # and Qian W. # (2023) Translational control of SOG1 expression in response to replication stress in Arabidopsis. Stress Biol. 3(1), 28.

3. Sun L.*, Cao Y.*, Li Z.*, Liu Y., Yin X., Deng X.W.#, He H.#, and Qian W.# (2023) Conserved H3K27me3-associated chromatin looping mediates physical interactions of gene clusters in plants. J. Integr. Plant Biol. 65, 1966-1982.

4. Li J. *, Wang C. *, Liang W., Zhang J., Jiang C-K., Liu Y., Ren Z., Ci D., Chang J., Han S., Deng X.W., Wang Y. #, and Qian W. # (2023) Functional importance and divergence of plant apurinic/apyrimidinic endonucleases in somatic and meiotic DNA repair. Plant Cell, 35, 2316-2331.

5. Wang Y. *, Liu Y. *, Qu S., Liang W., Sun L., Ci D., Ren Z., Fan L-M., and Qian W. # (2022) Nitrogen starvation induces genome-wide activation of transposable elements in Arabidopsis. J. Integr. Plant Biol. 64, 2374-2384.

6. Cao Y., Wang J., Wu S., Yin X., Shu J., Dai X., Liu Y., Sun L., Zhu D., Deng XW., Ye K., and Qian W.# (2022) The small nucleolar RNA SnoR28 regulates plant growth and development by directing rRNA maturation. Plant Cell, 34, 4173-4190.

7. Liu N.*, Xu Y.*, Li Q., Cao Y., Yang D., Liu S, Wang X, Mi Y., Liu Y, Ding C., Liu Y., Tuan YW., Gao G., Chen J.#, Qian W.#, and Zhang X.# (2022) A lncRNA fine-tunes salicylic acid biosynthesis to balance plant immunity and growth. Cell Host Microbe, 30, 1124-1138.

8. Tong J.*, Ren Z.*, Sun L.*, Zhou S., Yuan W., Hui Y., Ci D., Wang W., Fan L-M., Wu Z., and Qian W. # (2022) ALBA proteins confer thermotolerance through stabilizing HSF mRNAs in cytoplasmic granules. Nat. Plants, 8, 778-791.

9. Li J.*, Liang W.*, Liu Y., Ren Z., Ci D., Chang J., and Qian W.# (2022) The Arabidopsis ATR-SOG1 signaling module regulates pleiotropic developmental adjustments in response to 3'-blocked DNA repair intermediates. Plant Cell, 34, 852-866.

10. Liang W.*, Li J.*, Sun L.*, Liu Y., Lan Z., and Qian W.# (2022) Deciphering the synergistic and redundant roles of CG and non-CG DNA methylation in plant development and TE silencing. New Phytol. 233, 722-737.

11. Lin W.*, Sun L.*, Huang R., Liang W., Liu X., He H., Fukuda H., He X.Q.#, and Qian W.# (2020) Active DNA demethylation regulates tracheary element differentiation in Arabidopsis. Sci. Adv. 6, eaaz963.

12. Sun L.*, Jing Y.*, Liu X.*, Li Q.*, Xue Z., Cheng Z., Wang D., He H.#, and Qian W.# (2020) Heat stress-induced transposon activation correlates with 3D chromatin organization rearrangement in Arabidopsis. Nat. Commun. 11(1):1886. doi: 10.1038/s41467-020-15809-5.

13. Yuan, W.*, Zhou, J.*, Tong, J.*, Zhuo, W., Wang, L., Li, Y., Sun, Q.#, and Qian, W.# (2019). ALBA protein complex reads genic R-loops to maintain genome stability in Arabidopsis. Sci. Adv. 5, eaav9040.

14. Wang, X., Chen, X., Sun, L., and Qian, W. # (2019). Canonical cytosolic iron-sulfur cluster assembly and non-canonical functions of DRE2 in Arabidopsis. PLoS Genet. 15, e1008094.

15. Li, J.*, Liang, W.*, Li, Y., and Qian, W. # (2018). APURINIC/APYRIMIDINIC ENDONUCLEASE2 and ZINC FINGER DNA 3'-PHOSPHOESTERASE play overlapping roles in the maintenance of epigenome and genome stability. Plant Cell 30, 1954-1970.

16. Li, Y.*, Kumar, S.*, and Qian, W.# (2018). Active DNA demethylation: mechanism and role in plant development. Plant Cell Rep. 37, 77-85.

17. Jing, Y. *, Sun, H. *, Yuan, W., Wang, Y., Li, Q., Liu, Y., Li, Y., and Qian, W. # (2016). SUVH2 and SUVH9 couple two essential steps for transcriptional gene silencing in Arabidopsis. Mol. Plant 9, 1156-1167.

18. Wang, X.*, Li, Q.*, Yuan, W., Cao, Z., Qi, B., Kumar, S., Li, Y., and Qian, W. # (2016). The cytosolic Fe-S cluster assembly component MET18 is required for the full enzymatic activity of ROS1 in active DNA demethylation. Sci. Rep. 6, 26443.

19. Li, Q.*, Wang, X.*, Sun, H., Zeng, J., Cao, Z., Li, Y., and Qian, W. # (2015). Regulation of active DNA demethylation by a Methyl-CpG-Binding domain protein in Arabidopsis thaliana. PLoS Genet. 11, e1005210.